[Diagnosis and epidemiological surveillance of influenza and respiratory syncytial virus infections: interest of multiplex PCR]. / Diagnostic et surveillance épidémiologique des infections grippales et à virus respiratoire syncytial: intérêt de la PCR multiplex.
Med Mal Infect
; 37(11): 728-33, 2007 Nov.
Article
em Fr
| MEDLINE
| ID: mdl-17560747
ABSTRACT
OBJECTIVE:
Respiratory infections require a rapid etiological diagnosis for efficient management of cases. We evaluated multiplex PCR used for the diagnosis and the epidemiological surveillance of influenza and respiratory syncytial virus (RSV) infections. PATIENTS ANDMETHODS:
Our study included 278 patients (mean age 37.2+/-22.9 years) with flu or flu-like syndromes, consulting physicians affiliated with the GROG Poitou-Charentes or hospitalized in the Poitiers teaching hospital. A multiplex PCR detecting A(H3), A(H1) and B influenza viruses, and RSV A and B, was performed with both a direct examination by immunofluorescence and cell-culture.RESULTS:
We diagnosed a viral infection in 139 (50.0%) patients 99 cases of influenza A(H3), 2 cases of influenza A(H1), 28 cases of influenza B and 11 cases of RSV infections. The diagnosis yield in GROG patients (52.3%) was significantly higher than that observed in hospitalized patients (34.5%) (P=0.04). All techniques were correlated in 61% of cases. The multiplex PCR yielded 22.3% more positive samples compared to the conventional techniques. All positive samples by conventional techniques were also positive by multiplex PCR. We observed a perfect correlation between viral types and subtypes determined by PCR and cell-culture.CONCLUSION:
Multiplex PCR is a sensitive technique allowing an efficient and rapid diagnosis of respiratory infections due to influenza and RSV.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
/
Reação em Cadeia da Polimerase
/
Vírus Sincicial Respiratório Humano
/
Infecções por Vírus Respiratório Sincicial
/
Influenza Humana
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Adolescent
/
Adult
/
Aged
/
Child
/
Humans
/
Middle aged
País/Região como assunto:
Europa
Idioma:
Fr
Revista:
Med Mal Infect
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
França