Dietary loading and aggrecanase-1/TIMP-3 expression in rat mandibular condylar cartilage.
J Orofac Pain
; 21(3): 232-8, 2007.
Article
em En
| MEDLINE
| ID: mdl-17717962
ABSTRACT
AIMS:
To examine the expression of aggrecanase-1 and a tissue inhibitor of metalloproteinases (TIMP-3) in the condylar cartilage of young rats and to determine their relationship during altered dietary loading at different time points after weaning.METHODS:
One hundred Sprague-Dawley rats were randomly assigned to 1 of 2 groups the soft-diet group, which served as the control group (n=50), or the hard-diet group, which served as the experimental group (n=50). Ten soft- and 10 hard-diet rats were killed at 6 hours, 12 hours, 24 hours, 48 hours, and 9 days after weaning (i.e., after initiation of diet change for hard-diet rats). The right-side temporomandibular joints (TMJs) were prepared for immunohistochemical staining. The cartilage from the left-side mandibular condyles of all 10 animals in each group was combined for Western blot analysis.RESULTS:
Immunohistochemical analysis revealed strong staining for aggrecanase-1 localized mainly in the chondrocytes of proliferative and upper hypertrophic cartilage zones at all time points in both groups. The immunohistological expression of aggrecanase-1 was significantly higher in the hard-diet group at 12 and 24 hours than in the soft-diet group. Strong staining for TIMP-3 was mainly localized in the chondrocytes of proliferative and upper hypertrophic zones at all time points in both groups. The expression of TIMP-3 in the hard-diet group was at a significantly lower level compared to the soft-diet group at 6 hours. Western blot analysis also showed time-related differences in aggrecanase-1 and TIMP-3, but there was no significant difference between the 2 groups.CONCLUSION:
The temporary change in aggrecanase-1 and TIMP-3 expression reflects the complex interaction of these enzymes in the physiologic range and cartilage response to altered dietary loading.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pró-Colágeno N-Endopeptidase
/
Articulação Temporomandibular
/
Cartilagem Articular
/
Inibidor Tecidual de Metaloproteinase-3
/
Dieta
/
Proteínas ADAM
/
Côndilo Mandibular
Limite:
Animals
Idioma:
En
Revista:
J Orofac Pain
Assunto da revista:
NEUROLOGIA
/
ODONTOLOGIA
/
PSICOFISIOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Finlândia