Circadian and masking control of migratory restlessness in Gambel's white-crowned sparrow (Zonotrichia leucophrys gambelii).

ABSTRACT

Avian migration is a seasonal activity that requires intricate timing on both an annual and daily basis. With increasing evidence for endogenous regulation of daily activities in migrant species, we tested whether a circadian oscillator may be involved with the expressions of daily locomotor activities and specific behaviors of the long-distance migrant, Gambel's white-crowned sparrow (Zonotrichia leucophrys gambelii). Our previous studies have identified both daytime and nighttime behavioral patterns under a photoperiod of 18L6D. In 2 separate trials, birds in the vernal migratory life-history stage were exposed to constant dim light, (DD)dim, and constant bright light, LL, while locomotor activity and behavioral observations were collected. Under (DD)dim, the daytime behaviors that included active and quiescent components observed under 18L6D were lost as migratory restlessness, the intense nighttime activity, persisted nonstop for 36.4 h. Furthermore, the specific behaviors of migratory restlessness that are normally confined to the dark phase of 18L6D, beak-up and beak-up flight, were expressed also during the subjective day of (DD)dim. Birds exposed to LL retained similar patterns of activity to the 18L6D condition for 3 days, after which they became arrhythmic. Behavioral observations of intense locomotor activity observed during the subjective night of LL revealed no beak-up and beak-up flight. Thus, the complete expression of migratory restlessness that includes beak-up and beak-up flight may not be regulated by a circadian oscillator but instigated by very low light intensity. Locomotor activity and associated daytime behaviors appear to be influenced by a circadian oscillator, given their persistent patterns under LL. Therefore, we suggest that the separate components of migratory behavior are regulated differentially by a circadian oscillator and ambient lighting conditions.