Nucleic acid beacons for long-term real-time intracellular monitoring.
Anal Chem
; 80(8): 3025-8, 2008 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-18321137
ABSTRACT
Real time intracellular monitoring of biological molecules inside living cells is important in many biomedical studies and reveals valuable information unobtainable by conventional molecular biology techniques. A variety of methods and molecular probes have been developed, but long term (from a few hours to days) intracellular monitoring with high sensitivity and selectivity is impossible and has not been accomplished. We have used locked nucleic acids (LNA) to engineer novel molecular beacons (MBs) for long-term intracellular monitoring. The LNA-MBs were made of a mixed LNA and DNA bases to have extremely high biostability. The new beacons were tested with MDA-MB-231 cancer cells and used effectively to monitor mRNA expression levels in real-time for 5-24 h. After 24 h inside living cells, the LNA-MBs were still functional, demonstrating a greatly enhanced stability enabling the measurement of intracellular gene expression over an extended period of time.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Nucleicos
/
Técnicas de Sonda Molecular
Limite:
Humans
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos