Generation of C57BL/6 knockout mice using C3H x BALB/c blastocysts.
Biotechniques
; 44(3): 413-6, 2008 Mar.
Article
em En
| MEDLINE
| ID: mdl-18361795
ABSTRACT
The International Mouse Knockout Consortium aims to generate a knockout mouse for every single gene on a C57BL/6 background. Our ability to generate such mice is hampered by the poor economics of producing blastocysts to achieve germline transmission of C57BL/6 embryonic stem (ES) cells. We demonstrate superior utility of (C3H x BALB/c)F1 blastocysts compared with BALB/c blastocysts, with blastocyst numbers and germline transmission from subsequent chimeras at a rate 2- to 3-fold higher than that produced with BALB/c blastocysts.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Blastocisto
/
Engenharia Genética
/
Camundongos Knockout
/
Embrião de Mamíferos
Limite:
Animals
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos