Metal-chelate affinity chromatography.
Curr Protoc Neurosci
; Chapter 5: Unit 5.10, 2001 May.
Article
em En
| MEDLINE
| ID: mdl-18428493
ABSTRACT
Recombinant proteins engineered to have six consecutive histidine residues on either the amino or carboxy terminus can be purified using a resin containing nickel ions (Ni(2+)) that have been immobilized by covalently attached nitrilotriacetic acid (NTA). This technique is know as metal-chelate affinity chromatography and can be performed using either native or denatured protein. This unit presents protocols for expression of histidine-tail fusion proteins and their purification in either native or denatured form (along with procedures for renaturation by either dialysis or solid-phase renaturation). Also provided are procedures for analysis of the purified produce and regeneration of the NTA resin.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quelantes
/
Cromatografia de Afinidade
/
Metais
Limite:
Animals
Idioma:
En
Revista:
Curr Protoc Neurosci
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Estados Unidos