Multiple displacement amplification for malaria parasite DNA.
J Parasitol
; 95(1): 253-5, 2009 Feb.
Article
em En
| MEDLINE
| ID: mdl-18601578
ABSTRACT
Multiple displacement amplification (MDA) using Phi29 has proved to be an efficient, high-fidelity method for whole genome amplification in many organisms. This project was designed to evaluate this approach for use with the malaria parasite Plasmodium falciparum. In particular, we were concerned that the AT richness and presence of contaminating human DNA could limit efficiency of MDA in this system. We amplified 60 DNA samples using phi29 and scored 14 microsatellites, 9 single-nucleotide polymorphisms (SNPs), and gene copy number at GTP-cyclohydrolase I both before and after MDA. We observed 100% concordance in 829 microsatellite genotypes and in 499 SNP genotypes. Furthermore, copy number estimates for the GTP-cyclohydrolase I gene were correlated (r(2) = 0.67) in pre- and postamplification samples. These data confirm that MDA permits scoring of a range of different types of polymorphisms in P. falciparum malaria and can be used to extend the life of valuable DNA stocks.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmodium falciparum
/
DNA de Protozoário
/
Técnicas de Amplificação de Ácido Nucleico
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Parasitol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos