An amperometric horseradish peroxidase inhibition biosensor for the determination of phenylhydrazine.
Anal Sci
; 24(7): 895-9, 2008 Jul.
Article
em En
| MEDLINE
| ID: mdl-18614832
ABSTRACT
An amperometric horseradish peroxidase (HRP) inhibition biosensor has been substantially constructed by the help of N,N-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS). The preparation steps and the biosensor response to phenylhydrazine were monitored by electrochemical impedance spectroscopy (EIS), cyclic voltammetry, and chronoamperometry. The proposed biosensor could be applied to determine phenylhydrazine in a 0.10 M phosphate buffer solution containing 1.2 mM hydroquinone and 0.50 mM H(2)O(2) by phenylhydrazine, inhibiting the catalytic activity of the HRP enzyme in the reduction of H(2)O(2). The system was optimized to realize a reliable determination of phenylhydrazine in the range of 2.5 x 10(-7) to 1.1 x 10(-6) M with a detection limit of 8.2 x 10(-8) M and a correlation coefficient of 0.999. The modified electrode displayed good reproducibility, sensitivity and stability for the determination of phenylhydrazine.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenil-Hidrazinas
/
Análise Espectral
/
Técnicas Biossensoriais
/
Peroxidase do Rábano Silvestre
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Anal Sci
Ano de publicação:
2008
Tipo de documento:
Article