[Identification of endophytic Actinomycete Lj20 from plant and its antifungal substances].
Wei Sheng Wu Xue Bao
; 48(7): 900-4, 2008 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-18837367
ABSTRACT
OBJECTIVE:
Endophytic actinomycete Lj20 with antifungal activity was isolated from the roots of capsicum plants. We identified Lj20 and synthesized its antifungal substances.METHODS:
Morphological, biological and biochemical characteristics, chemotaxonomy analysis and 16S rDNA sequences were used to identify Lj20. According to GC-MS extrapolation result, one of antifungal substances in the metabolites of Lj20 was chemically synthesized. The bioactivities were determined by mycelium growth inhibition method.RESULTS:
Lj20 belonged to Streptomyces sp. and was similar to Streptomyces rochei. The metabolites contained butylated hydroxytoluene and 3, 5-di-tert-butyl-4-hydroxybenzyl methyl ether. The median effective concentration (EC50) of these two compounds to Botrytis cinerea Pers. were 237.04 mg/L of the water and 186.48 mg/L of the water, respectively.CONCLUSION:
Lj20 was classified as Streptomyces rochei. Butylated hydroxytoluene and 3, 5-di-tert-butyl-4-hydroxybenzyl methyl ether had significant inhibition to the pathogen.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Capsicum
/
Actinobacteria
/
Antifúngicos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
Wei Sheng Wu Xue Bao
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
China