Genome analysis of small-ruminant lentivirus genotype E: a caprine lentivirus with natural deletions of the dUTPase subunit, vpr-like accessory gene, and 70-base-pair repeat of the U3 region.
J Virol
; 83(2): 1152-5, 2009 Jan.
Article
em En
| MEDLINE
| ID: mdl-18987157
ABSTRACT
The nucleotide sequence of the highly divergent small-ruminant lentivirus genotype E has been determined. The full genome consists of 8,418 nucleotides and lacks two large portions corresponding nearly to the entire dUTPase subunit of the pol and vpr-like accessory genes. Moreover, the 70-bp repeat of the U3 region of the long terminal repeat was observed to be deleted. Interestingly, this lentivirus genotype is able to persist in a local breed population, and retrospective analysis revealed its presence in milk samples collected in 1999. gag sequences obtained from a flock coinfected with the B1 and E genotypes revealed that the evolutionary rates of the two viruses were quite similar. Since a reduced viral load and/or disease progression was observed for viruses with artificially deleted dUTPase and vpr-like genes, it is proposed that this viral cluster be designated a low-pathogenicity caprine lentivirus.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pirofosfatases
/
Proteínas Virais
/
Genoma Viral
/
Produtos do Gene vpr
/
Lentivirus Ovinos-Caprinos
/
Sequências Repetidas Terminais
Limite:
Animals
Idioma:
En
Revista:
J Virol
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Itália