DNA Checkerboard method for bacterial detection of microbiota from teeth and tongue biofilms. A preliminary study.
Minerva Stomatol
; 57(11-12): 561-7, 2008.
Article
em En
| MEDLINE
| ID: mdl-19092752
ABSTRACT
AIM:
The aim of this study was to determine the prevalence of 13 bacterial species in the dorsum tongue biofilm and compare its microbiota to that of subgingival samples in healthy adult subjects using DNA Checkerboard hybridization.METHODS:
A total of 40 samples, from subgingival and tongue biofilm, were enrolled into the study. All the samples were harvested and analyzed by DNA Checkerboard method for their content of 13 species of bacteria. Bacterial scores from each subject and location were averaged and the results of both sites were compared using the Wilcoxon signed ranks test.RESULTS:
All the microorganisms evaluated were detected in both tongue and subgingival samples, without significant differences in their prevalence.CONCLUSIONS:
The dorsum of the tongue was colonized by various microorganisms with similar prevalence as found in the subgingival biofilm of healthy individuals, suggesting that the tongue could be a potential microbial reservoir and may play a role in the recolonization of teeth surfaces.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Língua
/
Dente
/
DNA Bacteriano
/
Southern Blotting
/
Biofilmes
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Risk_factors_studies
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Minerva Stomatol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Brasil