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The Staphylococcus aureus protein Sbi acts as a complement inhibitor and forms a tripartite complex with host complement Factor H and C3b.
Haupt, Katrin; Reuter, Michael; van den Elsen, Jean; Burman, Julia; Hälbich, Steffi; Richter, Julia; Skerka, Christine; Zipfel, Peter F.
Afiliação
  • Haupt K; Department of Infection Biology, Leibniz Institute for Natural Product Research and Infection Biology, Jena, Germany.
PLoS Pathog ; 4(12): e1000250, 2008 Dec.
Article em En | MEDLINE | ID: mdl-19112495
The Gram-positive bacterium Staphylococcus aureus, similar to other pathogens, binds human complement regulators Factor H and Factor H related protein 1 (FHR-1) from human serum. Here we identify the secreted protein Sbi (Staphylococcus aureus binder of IgG) as a ligand that interacts with Factor H by a-to our knowledge-new type of interaction. Factor H binds to Sbi in combination with C3b or C3d, and forms tripartite SbiratioC3ratioFactor H complexes. Apparently, the type of C3 influences the stability of the complex; surface plasmon resonance studies revealed a higher stability of C3d complexed to Sbi, as compared to C3b or C3. As part of this tripartite complex, Factor H is functionally active and displays complement regulatory activity. Sbi, by recruiting Factor H and C3b, acts as a potent complement inhibitor, and inhibits alternative pathway-mediated lyses of rabbit erythrocytes by human serum and sera of other species. Thus, Sbi is a multifunctional bacterial protein, which binds host complement components Factor H and C3 as well as IgG and beta(2)-glycoprotein I and interferes with innate immune recognition.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Complemento C3b / Proteínas de Transporte / Fator H do Complemento / Inativadores do Complemento / Multimerização Proteica Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: PLoS Pathog Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Complemento C3b / Proteínas de Transporte / Fator H do Complemento / Inativadores do Complemento / Multimerização Proteica Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: PLoS Pathog Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Alemanha