[Lethal effect of cytotoxin from Agkitrodon acutus venom on gastric cancer cells and their ultrastructure changes].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 25(4): 335-7, 2009 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-19351505
ABSTRACT
AIM:
To purify cytotoxin from Agkitrodon acutus venom, and study the lethal effect of cytotoxin on gastric cancer cells and their ultrastructure changes.METHODS:
The cytotoxin was isolated and purified with successive chromatography on DEAE-Sephadex A-50 and TSKgel Pheny columns from Agkitrodon acutus venom. The feasibility of BGC-823 cells assessed using the MTT assay. The ultrastructure of BGC-823 cells treated with the cytotoxin was observed with the scanning and transmission electron microscope.RESULTS:
The purified cytotoxin H1 killed the gastric cancer cells. The cancer cell membrane and nuclear envelope were broken, mitochondria were swollen and intracellular substances were leaked out.CONCLUSION:
The cytotoxin from Agkitrodon acutus venom can kill the gastric cancer cells effectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Venenos de Crotalídeos
/
Citotoxinas
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China