Gamma-radiation-induced phosphorylation of p53 on serine 15 is dose-dependent in MOLT-4 leukaemia cells.
Folia Biol (Praha)
; 55(2): 41-4, 2009.
Article
em En
| MEDLINE
| ID: mdl-19454177
ABSTRACT
Molecular indicators of the absorbed dose of ionizing radiation are powerful tools in biodosimetry. The studies reported here were undertaken with the motivation to find such a marker among the mo lecules involved in ataxia-telangiectasia mutated kinase- dependent signalling induced by ionizing radiation (ATM-kinase, checkpoint kinase-2, protein p53, and oncoprotein Mdm2). In our previous work on T-lymphocyte leukaemia MOLT-4 cells we described the mentioned molecules of ATM-dependent pathway and none of them showed a pronounced dosedependent response. Here we employed Western blotting and ELISA assay to investigate the response of post-translationally modified p53 (particularly phosphorylated on serine 15) after gamma-irradiation. We have found the amount of phosphorylated p53 to be homogenously increased after irradiation by the doses of 0.5 to 7.5 Gy. The dose-dependent response was pronounced especially after the doses up to 3.0 Gy. The presented data indicate that p53 phosphorylated on serine 15 might be used as a potential biodosimetric marker.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Serina
/
Proteína Supressora de Tumor p53
/
Raios gama
Limite:
Humans
Idioma:
En
Revista:
Folia Biol (Praha)
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
República Tcheca