Pronuclear DNA injection for the production of transgenic rats.
Methods Mol Biol
; 561: 73-88, 2009.
Article
em En
| MEDLINE
| ID: mdl-19504065
ABSTRACT
In the absence of germ-line-competent ES cells in the rat, pronuclear microinjection remains an essential tool to generate transgenic rat models. However, DNA microinjection procedures first developed for mouse do not provide scientists with satisfying results when applied to rat. Here we describe optimized procedures for rat with a special focus on rat embryo production, in vitro incubation and culture, DNA pronuclear injection, and the transfer of embryos into foster females.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Animais Geneticamente Modificados
/
Núcleo Celular
/
Ratos Sprague-Dawley
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Técnicas de Transferência de Genes
/
Transgenes
/
Microinjeções
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
França