VLDL lipolysis products increase VLDL fluidity and convert apolipoprotein E4 into a more expanded conformation.
J Lipid Res
; 51(6): 1273-83, 2010 Jun.
Article
em En
| MEDLINE
| ID: mdl-19965582
ABSTRACT
Our previous work indicated that apolipoprotein (apo) E4 assumes a more expanded conformation in the postprandial period. The postprandial state is characterized by increased VLDL lipolysis. In this article, we tested the hypothesis that VLDL lipolysis products increase VLDL particle fluidity, which mediates expansion of apoE4 on the VLDL particle. Plasma from healthy subjects was collected before and after a moderately high-fat meal and incubated with nitroxyl-spin labeled apoE. ApoE conformation was examined by electron paramagnetic resonance spectroscopy using targeted spin probes on cysteines introduced in the N-terminal (S76C) and C-terminal (A241C) domains. Further, we synthesized a novel nitroxyl spin-labeled cholesterol analog, which gave insight into lipoprotein particle fluidity. Our data revealed that the order of lipoprotein fluidity was HDL approximately LDL
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apolipoproteína E4
/
Lipólise
/
Lipoproteínas VLDL
Limite:
Humans
Idioma:
En
Revista:
J Lipid Res
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Índia