Multicolor real-time PCR genotyping of ABO system using displacing probes.
J Forensic Sci
; 55(1): 19-24, 2010 Jan.
Article
em En
| MEDLINE
| ID: mdl-20002276
ABSTRACT
Rapid and informative ABO genotyping has become increasingly popular in forensic use. We developed a multiplex real-time polymerase chain reaction (PCR) approach to genotype ABO major groups and subgroups. Seven differently fluorophor-labeled displacing probes for O(1)(261delG), A(261G), A(796C/803C), B(796A/803C), O(2) (802G>A), A(2) (1059delC), and A(2) (1009A>G) were combined in one or two PCRs to determine either ABO major groups or subgroups. The method correctly detected 13 reference DNA samples. A blind test of 237 samples resulted in complete agreement with their phenotypes, and 110 of these 237 samples as well as with PCR-SSP method. The whole analysis could be finished in less than 100 min at substantially low material cost and the template DNA ranging from 0.16 to 500 ng per reaction could be quantitatively detected. Despite the limited informativeness of ABO genotyping, the developed methods could find application in rapid and inexpensive screening of forensic settings.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sistema ABO de Grupos Sanguíneos
/
Sondas de DNA
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Clinical_trials
Limite:
Humans
Idioma:
En
Revista:
J Forensic Sci
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China