Transduction of RNA-directed DNA methylation signals to repressive histone marks in Arabidopsis thaliana.
EMBO J
; 29(2): 352-62, 2010 Jan 20.
Article
em En
| MEDLINE
| ID: mdl-20010696
ABSTRACT
RNA-directed modification of histones is essential for the maintenance of heterochromatin in higher eukaryotes. In plants, cytosine methylation is an additional factor regulating inactive chromatin, but the mechanisms regulating the coexistence of cytosine methylation and repressive histone modification remain obscure. In this study, we analysed the mechanism of gene silencing mediated by MORPHEUS' MOLECULE1 (MOM1) of Arabidopsis thaliana. Transcript profiling revealed that the majority of up-regulated loci in mom1 carry sequences related to transposons and homologous to the 24-nt siRNAs accumulated in wild-type plants that are the hallmarks of RNA-directed DNA methylation (RdDM). Analysis of a single-copy gene, SUPPRESSOR OF drm1 drm2 cmt3 (SDC), revealed that mom1 activates SDC with concomitant reduction of di-methylated histone H3 lysine 9 (H3K9me2) at the tandem repeats in the promoter region without changes in siRNA accumulation and cytosine methylation. The reduction of H3K9me2 is not observed in regions flanking the tandem repeats. The results suggest that MOM1 transduces RdDM signals to repressive histone modification in the core region of RdDM.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Proteínas Nucleares
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Arabidopsis
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RNA de Plantas
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Regulação da Expressão Gênica de Plantas
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Metilação de DNA
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Inativação Gênica
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Proteínas de Arabidopsis
Idioma:
En
Revista:
EMBO J
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Japão