[Study on the automatic ribotyping for Enterobacter sakazakii].
Zhonghua Yu Fang Yi Xue Za Zhi
; 43(10): 900-2, 2009 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-20137472
ABSTRACT
OBJECTIVE:
To analyze the ribotyping fingerprint of Enterobacter sakazakii (E. sakazakii) isolated from food and its typing power.METHODS:
Two standard strains and twenty-eight isolates of E.sakazakii were analyzed by the DuPont Riboprinter(TM) microbial characterization system. The relevant database was established and the fingerprint patterns were analyzed with BioNumerics software.RESULTS:
This system grouped two standard strains and twenty-eight E.sakazakii isolates into 26 ribotypes, and four ribotypes included two strains respectively, the other twenty-two strains showed different ribotypes. The lowest similarity was 31.58%. The number of bands by ribotyping was approximately ten and the molecular weight of these bands ranged from 1 to 50 kb. By the clustering program in BioNumerics, these isolates could be grouped into four clusters.CONCLUSION:
The automatic ribotyping method is convenient and fast in E.sakazakii typing.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ribotipagem
/
Cronobacter sakazakii
/
Microbiologia de Alimentos
Idioma:
Zh
Revista:
Zhonghua Yu Fang Yi Xue Za Zhi
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China