Rapid pretreatment-free immunochromatographic assay of chloramphenicol in milk.
Talanta
; 81(3): 843-8, 2010 May 15.
Article
em En
| MEDLINE
| ID: mdl-20298863
ABSTRACT
A pretreatment-free immunochromatographic assay for detection of chloramphenicol (CAP) in milk was developed. The assay is based on competition between CAP molecules in the sample and immobilized CAP-protein conjugate for binding to monoclonal anti-CAP antibodies conjugated with colloidal gold particles (average diameter 30nm). The assay is carried out in the course of sample flowing along test strip with immobilized reactants, and its results can be detected by the naked eye or by a photometric device. Effect of the concentration of immunoreactants on assay characteristics was studied. The assay protocol with maximal sensitivity and reliability was optimized using measured values of brightness of lines. Detection limit for CAP is 10ngmL(-1). Assay duration is 10min, and it can be carried out at room temperature without any additional devices and reactants. The developed test strip has been applied to CAP detection in dairy products.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoensaio
/
Cloranfenicol
/
Cromatografia
/
Leite
Limite:
Animals
Idioma:
En
Revista:
Talanta
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Federação Russa