The expression pattern of SEPT7 correlates with sperm morphology.
J Assist Reprod Genet
; 27(6): 299-307, 2010 Jun.
Article
em En
| MEDLINE
| ID: mdl-20352323
PURPOSE: To investigate the expression pattern of the SEPT7 protein during spermatogenesis and its potential role in sperm function. METHODS: We first investigated the expression pattern of SEPT7 during different steps of mouse spermiogenesis using an immunofluorescence assay (IFA). IFA was also applied to study the expression pattern of SEPT7 in human ejaculated spermatozoa. Nine fertile men with normal semen parameters were used as the control group, and 21 infertile men with asthenozoospermia were recruited as the patient group. We assessed the frequency of the SEPT7 signal in the various morphological subgroups. RESULTS: In humans, the frequency of a defective SEPT7 signal was significantly increased in men with asthenozoospermia. The absence of a SEPT7 signal was more prevalent in sperm containing morphological defects of various types. CONCLUSIONS: The expression pattern of SEPT7 suggested that this protein may be involved in the regulation of subcellular-compartment formation during spermiogenesis in the mouse. The absence of a SEPT7 signal correlated with multiple sperm defects.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Proteínas de Ligação ao GTP
/
Proteínas do Citoesqueleto
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
J Assist Reprod Genet
Assunto da revista:
GENETICA
/
MEDICINA REPRODUTIVA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Taiwan