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Protein-protein recognition control by modulating electrostatic interactions.
Han, Song; Yin, Shijin; Yi, Hong; Mouhat, Stéphanie; Qiu, Su; Cao, Zhijian; Sabatier, Jean-Marc; Wu, Yingliang; Li, Wenxin.
Afiliação
  • Han S; State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei, P. R. China.
J Proteome Res ; 9(6): 3118-25, 2010 Jun 04.
Article em En | MEDLINE | ID: mdl-20405930
ABSTRACT
Protein-protein control recognition remains a huge challenge, and its development depends on understanding the chemical and biological mechanisms by which these interactions occur. Here we describe a protein-protein control recognition technique based on the dominant electrostatic interactions occurring between the proteins. We designed a potassium channel inhibitor, BmP05-T, that was 90.32% identical to wild-type BmP05. Negatively charged residues were translocated from the nonbinding interface to the binding interface of BmP05 inhibitor, such that BmP05-T now used BmP05 nonbinding interface as the binding interface. This switch demonstrated that nonbinding interfaces were able to control the orientation of protein binding interfaces in the process of protein-protein recognition. The novel function findings of BmP05-T peptide suggested that the control recognition technique described here had the potential for use in designing and utilizing functional proteins in many biological scenarios.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Engenharia de Proteínas / Mapeamento de Interação de Proteínas / Domínios e Motivos de Interação entre Proteínas / Modelos Biológicos Tipo de estudo: Prognostic_studies Idioma: En Revista: J Proteome Res Assunto da revista: BIOQUIMICA Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeos / Engenharia de Proteínas / Mapeamento de Interação de Proteínas / Domínios e Motivos de Interação entre Proteínas / Modelos Biológicos Tipo de estudo: Prognostic_studies Idioma: En Revista: J Proteome Res Assunto da revista: BIOQUIMICA Ano de publicação: 2010 Tipo de documento: Article