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Two cyclin-dependent kinase pathways are essential for polarized trafficking of presynaptic components.
Ou, Chan-Yen; Poon, Vivian Y; Maeder, Celine I; Watanabe, Shigeki; Lehrman, Emily K; Fu, Amy K Y; Park, Mikyoung; Fu, Wing-Yu; Jorgensen, Erik M; Ip, Nancy Y; Shen, Kang.
Afiliação
  • Ou CY; Department of Biology, Howard Hughes Medical Institute, Stanford University, 385 Serra Mall, California 94305, USA.
Cell ; 141(5): 846-58, 2010 May 28.
Article em En | MEDLINE | ID: mdl-20510931
Polarized trafficking of synaptic proteins to axons and dendrites is crucial to neuronal function. Through forward genetic analysis in C. elegans, we identified a cyclin (CYY-1) and a cyclin-dependent Pctaire kinase (PCT-1) necessary for targeting presynaptic components to the axon. Another cyclin-dependent kinase, CDK-5, and its activator p35, act in parallel to and partially redundantly with the CYY-1/PCT-1 pathway. Synaptic vesicles and active zone proteins mostly mislocalize to dendrites in animals defective for both PCT-1 and CDK-5 pathways. Unlike the kinesin-3 motor, unc-104/Kif1a mutant, cyy-1 cdk-5 double mutants have no reduction in anterogradely moving synaptic vesicle precursors (SVPs) as observed by dynamic imaging. Instead, the number of retrogradely moving SVPs is dramatically increased. Furthermore, this mislocalization defect is suppressed by disrupting the retrograde motor, the cytoplasmic dynein complex. Thus, PCT-1 and CDK-5 pathways direct polarized trafficking of presynaptic components by inhibiting dynein-mediated retrograde transport and setting the balance between anterograde and retrograde motors.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sinapses / Quinases Ciclina-Dependentes / Proteínas de Caenorhabditis elegans Limite: Animals Idioma: En Revista: Cell Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sinapses / Quinases Ciclina-Dependentes / Proteínas de Caenorhabditis elegans Limite: Animals Idioma: En Revista: Cell Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Estados Unidos