Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels.
Int Endod J
; 43(5): 430-5, 2010 May.
Article
em En
| MEDLINE
| ID: mdl-20518937
ABSTRACT
AIM:
To evaluate the mechanisms of cytotoxicity of chlorhexidine (CHX) in human osteoblastic cells in vitro.METHODOLOGY:
Cytotoxicity, cell proliferation and collagen synthesis assays were performed to elucidate the toxic effects of CHX on the human osteoblastic cell line U2OS. To determine whether glutathione (GSH) levels were important in the cytotoxicity of CHX, cells were pre-treated with 2-oxothiazolidine-4-carboxylic acid (OTZ) to boost GSH levels or buthionine sulfoximine (BSO) to deplete GSH.RESULTS:
CHX demonstrated a cytotoxic effect to U2OS cells in a dose-dependent manner (P < 0.05). The 50% inhibition concentration of CHX was approximately 0.005%. CHX also inhibited cell proliferation and collagen synthesis (P < 0.05). The addition of OTZ acted as a protective effect on the CHX-induced cytotoxicity (P < 0.05). In contrast, the addition of BSO enhanced the CHX-induced cytotoxicity (P < 0.05).CONCLUSIONS:
The levels of CHX tested inhibited cell growth, proliferation and collagen synthesis on U2OS cells. CHX has significant potential for periapical toxicity. GSH depletion might be one of the mechanisms underlying CHX cytotoxicity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Clorexidina
/
Glutationa
/
Anti-Infecciosos Locais
Limite:
Humans
Idioma:
En
Revista:
Int Endod J
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Taiwan