Selected ion monitoring gas chromatography/mass spectrometry using uniformly labelled (13C)glucose for determination of glucose turnover in man.
Biol Mass Spectrom
; 20(4): 203-9, 1991 Apr.
Article
em En
| MEDLINE
| ID: mdl-2054393
ABSTRACT
An optimized method is described for the mass fragmentographic determination of uniformly labelled (13C)glucose in human plasma using a butylboronic acid acetate derivative, and capillary gas chromatography. The advantages of the method are the ease and speed of the derivatization procedure, the small sample size, high precision (interassay coefficient of variation 5.7%), and applicability of a relatively low-cost mass spectrometer. This method allows glucose tracer experiments to be performed in man using the bolus injection technique, necessitating analysis of many samples. The results on glucose turnover obtained in a clinical experiment were in full agreement with previously published data.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicemia
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Biol Mass Spectrom
Assunto da revista:
BIOQUIMICA
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Alemanha