Bisulfite sequencing of DNA.
Curr Protoc Mol Biol
; Chapter 7: Unit 7.9.1-17, 2010 Jul.
Article
em En
| MEDLINE
| ID: mdl-20583099
ABSTRACT
Exact positions of 5-methylcytosine (m(5)C) on a single strand of DNA can be determined by bisulfite genomic sequencing (BGS). Treatment with bisulfite ion preferentially deaminates unmethylated cytosines, which are then converted to uracil upon desulfonation. Amplifying regions of interest from deaminated DNA and sequencing products cloned from amplicons permits determination of methylation at single-nucleotide resolution along single DNA molecules, which is not possible with other methylation analysis techniques. This unit describes a BGS technique suitable for most DNA sources, including formaldehyde-fixed tissue. Considerations for experimental design and common sources of error are discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sulfitos
/
DNA
/
Análise de Sequência de DNA
/
5-Metilcitosina
Idioma:
En
Revista:
Curr Protoc Mol Biol
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Estados Unidos