Humic substances induce lateral root formation and expression of the early auxin-responsive IAA19 gene and DR5 synthetic element in Arabidopsis.
Plant Biol (Stuttg)
; 12(4): 604-14, 2010 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-20636903
ABSTRACT
Humic substances (HS) have positive effects on plant physiology, but the molecular mechanisms underlying these events are only partially understood. HS exert auxin-like activity, but data supporting this hypothesis are under debate. To investigate the auxin-like activity of HS, we studied their biological effect on lateral root initiation in Arabidopsis thaliana. To this aim we characterised HS by means of DRIFT and (13)C CP/MAS NMR spectroscopy, and measured their endogenous content of IAA. We then utilised a combination of genetic and molecular approaches to unravel HS auxin activity in the initiation of lateral roots. The data obtained using specific inhibitors of auxin transport or action showed that HS induce lateral root formation mostly through their 'auxin activity'. These findings were further supported by the fact that HS used in this study activated the auxin synthetic reporter DR5GUS and enhanced transcription of the early auxin responsive gene IAA19.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arabidopsis
/
Raízes de Plantas
/
Proteínas de Arabidopsis
/
Substâncias Húmicas
Idioma:
En
Revista:
Plant Biol (Stuttg)
Assunto da revista:
BOTANICA
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Itália