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Expression of Notch signalling-related genes in normal and differentiating rat dental pulp cells.
Sun, Hantang; Kawashima, Nobuyuki; Xu, Jing; Takahashi, Satomi; Suda, Hideaki.
Afiliação
  • Sun H; Department of Operative Dentistry and Endodontics, School of Stomatology, the Fourth Military Medical University, Xi'an, Shaanxi, China.
Aust Endod J ; 36(2): 54-8, 2010 Aug.
Article em En | MEDLINE | ID: mdl-20666749
Notch signalling is of fundamental importance to various processes during embryonic development and in adults. The possible role of Hey1, an important Notch signalling component, in odontoblast differentiation was evaluated in this study. Primary cultured dental pulp cells, derived from upper incisors of 5-week-old Wistar rats, were placed in alpha-modification of Eagle's minimal essential medium supplemented with 10% Fetal Bovine Serum (FBS), and ascorbic acid (AA) and beta-glycerophosphate (beta-GP), with or without dexamethasone, and cultured on dishes coated with collagen type IA for 7 days. Conventional and real-time Polymerase Chain Reaction (PCR) was performed to determine the expression of Notch-related genes and dentin sialophosphoprotein as a marker of odontoblast differentiation. Dentin sialophosphoprotein and Hey1 expression was significantly increased and decreased in the presence of AA + beta-GP compared with controls, respectively. These findings suggest that Hey1 may be a negative regulator in odontoblast differentiation.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Transdução de Sinais / Sequências Hélice-Alça-Hélice / Polpa Dentária / Fatores de Transcrição Hélice-Alça-Hélice Básicos / Receptores Notch Limite: Animals Idioma: En Revista: Aust Endod J Assunto da revista: ODONTOLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Transdução de Sinais / Sequências Hélice-Alça-Hélice / Polpa Dentária / Fatores de Transcrição Hélice-Alça-Hélice Básicos / Receptores Notch Limite: Animals Idioma: En Revista: Aust Endod J Assunto da revista: ODONTOLOGIA Ano de publicação: 2010 Tipo de documento: Article País de afiliação: China