[Study on sensitization and mechanism of CGA-BSA].
Zhongguo Zhong Yao Za Zhi
; 35(9): 1181-4, 2010 May.
Article
em Zh
| MEDLINE
| ID: mdl-20707079
ABSTRACT
OBJECTIVE:
To investigate the sensitization and mechanism of artificial antigen of chlorogenic acid (CGA-BSA).METHOD:
Using intensive immunization to establish allergy animal model on guinea pig and preparing antiserum and tissue for further test. Using HE staining to observe pathology change of lungs, trachea, liver. Using passive mast cell (PMC) degranulation test to observe the immunogenicity of CGA-BSA and using ELISA to detect IgE and histamine in plasma.RESULT:
There established allergy animal model on guinea pig, which include a increase cell degranulation by a ratio (63.58 +/- 10.23)% in PMC test, increase of specific antibody IgE and increase of histamine in plasma after provocation by ELISA.CONCLUSION:
Allergen CGA-BSA could provoke allergenic response in guinea pig, and the allergic response belongs to type I allergy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Soroalbumina Bovina
/
Medicamentos de Ervas Chinesas
/
Ácido Clorogênico
/
Hipersensibilidade
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
China