An expanding negative detection method for the visualization of DNA in polyacrylamide gels by eosin Y.
Electrophoresis
; 31(20): 3450-6, 2010 Oct.
Article
em En
| MEDLINE
| ID: mdl-20879043
ABSTRACT
A sensitive and easy technique has been developed for the negative detection of DNA following PAGE using eosin Y. After electrophoresis, gels are fixed and stained within 40 min to provide a detection limit of 0.1-0.2 ng of single DNA band, which appears as transparent and colorless under the opaque gel matrix background. The sensitivity of the new stain is fourfold better than zinc-imidazole negative and ethidium bromide stains. Furthermore, the newly developed staining method has been successfully applied to RNA visualization in polyacrylamide gels. In addition, the inclusion of inorganic salts in staining solution was also investigated, which has great effect on the staining efficiency.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Coloração Negativa
/
Amarelo de Eosina-(YS)
/
Eletroforese em Gel de Poliacrilamida
/
Corantes Fluorescentes
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
2010
Tipo de documento:
Article