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8-oxo-7,8-dihydro-2'-deoxyguanosine as a biomarker of oxidative damage in oesophageal cancer patients: lack of association with antioxidant vitamins and polymorphism of hOGG1 and GST.
Lagadu, Stéphanie; Lechevrel, Mathilde; Sichel, François; Breton, Jean; Pottier, Didier; Couderc, Rémy; Moussa, Fathi; Prevost, Virginie.
Afiliação
  • Lagadu S; Groupe Régional d'Etudes sur le Cancer-UPRES EA 1772-IFR 146, Université de Caen-Basse-Normandie and Centre de Lutte Contre le Cancer François Baclesse, Caen, France.
J Exp Clin Cancer Res ; 29: 157, 2010 Dec 06.
Article em En | MEDLINE | ID: mdl-21134244
BACKGROUND: The present report was designed to investigate the origins of elevated oxidative stress measured in cancer patients in our previous work related to a case-control study (17 cases, 43 controls) on oesophageal cancers. The aim was to characterize the relationship between the levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), antioxidant vitamins and genetic susceptibility. METHODS: 8-oxodG was analysed in peripheral blood mononuclear cells (PBMCs) by High Performance Liquid Chromatography with Electrochemical Detection (HPLC-ED). Analysis of gene polymorphisms in GSTM1 and GSTT1 was performed by multiplex PCR and in GSTP1 and hOGG1 by a PCR-RFLP method. Reversed-phase HPLC with UV detection at 294 nm was used to measure vitamins A and E in serum from the same blood samples. RESULTS: We observed that in our combined population (cases and control, n = 60), there was no statistically significant correlation between the levels of 8-oxodG and (i) the serum concentration of antioxidant vitamins, vitamin A (P = 0.290) or vitamin E (P = 0.813), or (ii) the incidence of the Ser326Cys polymorphic variant (P = 0.637) of the hOGG1 gene. Also, the levels of 8-oxodG were not significantly associated with polymorphisms in metabolite-detoxifying genes, such as GSTs, except for the positive correlation with Val/Val GST P1 allele (P < 0.0001). CONCLUSIONS: The weakness of our cohort size notwithstanding, vitamins levels in serum and genetic polymorphisms in the hOGG1 or GST genes do not appear to be important modulators of 8-oxodG levels.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Esofágicas / Biomarcadores Tumorais / Predisposição Genética para Doença / Desoxiguanosina Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: J Exp Clin Cancer Res Ano de publicação: 2010 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Esofágicas / Biomarcadores Tumorais / Predisposição Genética para Doença / Desoxiguanosina Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: J Exp Clin Cancer Res Ano de publicação: 2010 Tipo de documento: Article País de afiliação: França