Neutrophil defensins: their possible role in allergic asthma.
J Investig Allergol Clin Immunol
; 21(1): 38-43, 2011.
Article
em En
| MEDLINE
| ID: mdl-21370722
ABSTRACT
BACKGROUND:
Neutrophil defensins, originally identified as broad-spectrum antimicrobial peptides, have been implicated in the regulation of inflammatory and immunological processes.OBJECTIVES:
To investigate whether the in vitro challenge of neutrophils from patients with bronchial asthma with allergens stimulated the release of alpha-defensins and whether levels released were dependent on lung infections.METHOD:
The neutrophils were cultivated with different agonists and the concentration of alpha-defensin in cell-free supernatant was measured with enzyme-linked immunosorbent assay (ELISA).RESULTS:
Neutrophils from allergic patients released alpha-defensins via an allergen-dependent mechanism. Our results indicate that the in vitro activation of neutrophils is highly allergen-specific. In this context, allergens other than those which produced clinical symptoms did not elicit alpha-defensin release, and allergens had no effect on neutrophils from healthy donors. However, neutrophils from both allergic patients and healthy controls were able to release alpha-defensins upon treatment with PMA. In the allergen-stimulated neutrophils, cells from asthmatic patients stimulated with a sensitizing allergen showed a significantly higher production of alpha-defensin under respiratory tract infection than cells from the same patients without such an infection.CONCLUSION:
Neutrophils from allergic patients release alpha-defensins via an allergen-dependent mechanism.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Asma
/
Alérgenos
/
Alfa-Defensinas
/
Neutrófilos
Limite:
Adult
/
Humans
Idioma:
En
Revista:
J Investig Allergol Clin Immunol
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Espanha