SLA-DRB1 and -DQB1 genotyping by the PCR-SSOP-Luminex method.
Tissue Antigens
; 78(1): 49-55, 2011 Jul.
Article
em En
| MEDLINE
| ID: mdl-21506937
ABSTRACT
A simple and novel genotyping method was developed to detect alleles at the swine leukocyte antigen (SLA)-DRB1 and -DQB1 class II loci by using polymerase chain reaction (PCR)-fluorescently labeled sequence-specific oligonucleotide probes (SSOPs) and Luminex 100 xMAP detection. The PCR-SSOP-Luminex method exhibited accuracy of 95% for both SLA-DRB1 and -DQB1 in 6 homozygous and 16 heterozygous pig samples as confirmed by sequencing the PCR products of the same samples. In addition, 12 low-resolution SLA class II haplotypes consisting of 7 and 9 DRB1 and DQB1 alleles were identified, respectively, in one population of 283 Landrace pigs. This genotyping method facilitates the rapid and accurate identification of two- or four-digit alleles at the SLA-DRB1 and -DQB1 loci.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Suínos
/
Antígenos de Histocompatibilidade Classe II
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Tissue Antigens
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
Japão