[Effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells of children with febrile seizures].
Zhongguo Dang Dai Er Ke Za Zhi
; 13(4): 285-7, 2011 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-21507295
ABSTRACT
OBJECTIVE:
To study the effects of TNF-α on ICAM-1 and LFA-1 expression in peripheral blood mononuclear cells (PBMC) of children with febrile seizures (FS).METHODS:
Sixteen children with FS and 16 age- and gender-matched healthy children were enrolled. The samples of PBMC from FS children were randomized into two groups with or without TNF-α treatment (TNF-α concentration 1.0 ng/mL). PBMC were purified and cultured with a conventional method in vitro. The expression of ICAM-1 and LFA-1 in PBMC was determined by flow cytometry (FCM).RESULTS:
ICAM-1ï¼»(20±9)% vs (14±7)%)ï¼½and LFA-1ï¼»(43±16)% vs (30±16)%ï¼½expression in PBMC in the untreated FS group was significantly higher than that in the normal control group (P<0.05). Compared with the untreated FS group, the treatment with TNF-α remarkably increased the ICAM-1 expressionï¼»(27±11)%ï¼½(P<0.05). PBMC LFA-1 expressionï¼»(52±21)%ï¼½in the TNF-α-treated group was higher than that in the untreated FS group, although there were no statistical differences between the two groups.CONCLUSIONS:
TNF-α treatment may increase LFA-1 and ICAM-1 expression in PBMC of children with FS.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucócitos Mononucleares
/
Antígeno-1 Associado à Função Linfocitária
/
Fator de Necrose Tumoral alfa
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Molécula 1 de Adesão Intercelular
/
Convulsões Febris
Tipo de estudo:
Clinical_trials
Limite:
Child
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Child, preschool
/
Female
/
Humans
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Infant
/
Male
Idioma:
Zh
Revista:
Zhongguo Dang Dai Er Ke Za Zhi
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China