[Effects of injectable chitosan thermosensitive hydrogel on dog bone marrow stromal cells in vitro].

ABSTRACT

PURPOSE: To investigate the biological effects of injectable chitosan(CS) thermosensitive hydrogel on dog bone marrow stromal cells(BMSCs) in vitro. METHODS: Dog BMSCs were obtained from dog bone marrow aspiration by density gradient centrifugation method, cultured in DMEM medium with 10% fetal bovine serum (FBS) and identified. Chitosan thermosensitive hydrogel was prepared and the BMSCs cultured in a medium containing hydrogel were observed under light microscope. The extract liquid from hydrogel was made and its effects on cell proliferation and differentiation were evaluated. Statistical significance was assessed with SPSS 13.0 software package for one -way ANOVA. RESULTS: Dog BMSCs survived in the extract liquid and there was no significant difference. The proliferation of BMSCs in mineralization condition medium was lower than normal medium after 1,4,7 days culture and was similar to that in normal medium after 10 days. The osteogenic extract liquid improved the secretion of alkaline phosphatase (ALP) and osteocalcin (OC). CONCLUSIONS: The CS thermosensitive hydrogel possesses good biocompatibility and can be used for in vitro and in vivo experimental studies of CS hydrogel loaded with BMSCs.