Matrix-assisted laser desorption/ionization-post source decay fragmentation of cystine- containing amphibian peptides with novel cysteine tags.
Eur J Mass Spectrom (Chichester)
; 17(1): 73-83, 2011.
Article
em En
| MEDLINE
| ID: mdl-21625031
ABSTRACT
Long disulphide-containing peptides brevinins 1E and 2Ec from the skin secretion of the frog Rana ridibunda were reduced and alkylated with ten novel and three known derivatizing agents. Nine of novel reagents are maleimide derivatives. The peptides were also reduced with DTT directly onto the MALDI target without alkylation. Modified samples were subjected to MALDI-PSD study. Procedures, fragmentation patterns, fragment ion signal abundances and sequence coverage for two peptides modified with thirteen tags (or on-plate reduced) are described. The fast on-plate procedure for reduction/alkylation was applied to Rana ridibunda crude secretion, providing intensive signals of derivatized peptides. The corresponding ions may be used for the MS/MS sequencing procedure.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Rana ridibunda
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
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Peptídeos Catiônicos Antimicrobianos
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Cistina
/
Proteínas de Anfíbios
Limite:
Animals
Idioma:
En
Revista:
Eur J Mass Spectrom (Chichester)
Ano de publicação:
2011
Tipo de documento:
Article