Amplification of the c-myc gene in human medulloblastoma cell lines and xenografts.
Cancer Res
; 50(8): 2347-50, 1990 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-2180567
ABSTRACT
Cultured cell lines and xenografts derived from 7 human medulloblastomas were evaluated for amplification of the c-myc, N-myc, epidermal growth factor receptor, and gli genes by Southern blot analysis. Karyotypes of the original biopsies and early passaged cells demonstrated double minute chromosomes in 4 of the 7 cases. All 7 samples (3 cell lines and 4 xenografts) from the 4 tumors with double minute chromosomes contained amplification of the c-myc gene. Cell lines and xenografts derived from the 3 biopsies without double minute chromosomes failed to demonstrate amplification of the 4 genes which were tested, but a rearrangement of the c-myc gene occurred in 1 of the 3 tumors. These observations demonstrate that the c-myc gene is often amplified and/or rearranged in human medulloblastomas and suggest that amplification of this gene provides a growth advantage for medulloblastoma cells in vitro and in vivo.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proto-Oncogenes
/
Neoplasias Cerebelares
/
Amplificação de Genes
/
Proteínas Proto-Oncogênicas
/
Meduloblastoma
Limite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1990
Tipo de documento:
Article