[Effect of calcitonin gene-related peptide on RUNX2 expression in primary rat osteoblasts].
Beijing Da Xue Xue Bao Yi Xue Ban
; 43(5): 652-6, 2011 Oct 18.
Article
em Zh
| MEDLINE
| ID: mdl-22008669
ABSTRACT
OBJECTIVE:
To investigate the effects of exogenous calcitonin gene-related peptide (CGRP) on RUNX2 expression in primary cultured rat osteoblasts.METHODS:
Primary cultured rat osteoblasts were obtained by collagenase / trypsinenzyme digestion from calvarial bone of neonate rats and were identified by ALP staining; The cells were exposed to gradient concentrations, and the effect of CGRP on the proliferation of osteoblasts was determined by MTT assay. The expressions of RUNX2 were quantified by RT-PCR and Western blotting after 48-hour exposure to different CGRP concentrations.RESULTS:
CGRP could promote the proliferation of osteoblasts .When the concentration of CGRP were at 10(-8)-10(-6) mol/L, the proliferation rates of osteoblasts were 71.9%, 142.1%, 321.0%, respectively (P<0.05); when CGRP was at concentrations of 10(-7) and 10(-6) mol /L in osteoblasts for 48 h, the mRNA levels of RUNX2 was significantly increased, which increased (46.2±11.2)% and (58.6±14.0)% respectively (P<0.05); the expression of RUNX2 protein levels was consistent with the change of the mRNA levels.CONCLUSION:
CGRP could promote RUNX2 expression in primary cultured rat osteoblasts, which suggests that RUNX2 is involved in the mechanism of osteoblast proliferation induced by CGRP.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Peptídeo Relacionado com Gene de Calcitonina
/
Subunidade alfa 1 de Fator de Ligação ao Core
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Beijing Da Xue Xue Bao Yi Xue Ban
Assunto da revista:
MEDICINA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China