[Study on correlation between ITS sequence of Arctium lappa and quality of Fructus Arctii].

ABSTRACT

OBJECTIVE: To study the correlation between ITS sequence of Arctium lappa and Fructus Arctii quality of different origin. METHOD: The samples of Fructu arctii materials were collected from 26 different producing areas. Their ITS sequence were determined after polymerase chain reaction (PCR) and quality were evaluated through the determination of arctiin content by HPLC. Genetic diversity, genotype and correlation were analyzed by ClustalX (1.81), Mage 4.0, SPSS 13.0 statistical software. RESULT: ITS sequence of A. was obtained from 26 samples, and was registered in the GenBank. Corresponding arctiin content of Fructus arctii and 1000-grain weight were determined. A. lappa genotype correlated with Fructus arctii quality by statistical analysis. CONCLUSION: The research provided a foundation for revealing the molecular mechanism of Fructus arctii geoherbs.