[TSA improve transgenic porcine cloned embryo development and transgene expression].
Yi Chuan
; 33(7): 749-56, 2011 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-22049689
ABSTRACT
Uncompleted epigenetic reprogramming is attributed to the low efficiency of producing transgenic cloned animals. Histone modification associated with epigenetics can directly influence the embryo development and transgene expression. Trichostatin A (TSA), as an inhibitor of histone deacetylase, can change the status of histone acetylation, improve somatic cell reprogramming, and enhance cloning efficiency. TSA prevents the chromatin structure from being condensed, so that transcription factor could binds to DNA sequence easily and enhance transgene expression. Our study established the optimal TSA treatment on porcine donor cells and cloned embryos, 250 nmol/L, 24 h and 40 nmol/L, 24 h, respectively. Furthermore, we found that both the cloned embryo and the donor cell treated by TSA resulted in the highest development efficiency. Meanwhile, TSA can improve transgene expression in donor cell and cloned embryo. In summary, TSA can significantly improve porcine reconstructed embryo development and transgene expression.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Suínos
/
Transgenes
/
Desenvolvimento Embrionário
/
Inibidores de Histona Desacetilases
/
Ácidos Hidroxâmicos
Limite:
Animals
/
Pregnancy
Idioma:
Zh
Revista:
Yi Chuan
Assunto da revista:
GENETICA
Ano de publicação:
2011
Tipo de documento:
Article
País de afiliação:
China