Your browser doesn't support javascript.
loading
[High-throughput screening of human pancreatic alpha-amylase inhibitors].
Qi, Xizhen; Ren, Limei; Zheng, Fang; Zhang, Qi; Bai, Fang; Bai, Gang.
Afiliação
  • Qi X; College of Pharmacy, Nankai University, Tianjin 300071, China. qixizhen@163.com
Wei Sheng Wu Xue Bao ; 51(8): 1106-12, 2011 Aug.
Article em Zh | MEDLINE | ID: mdl-22097776
OBJECTIVE: Targeting the important enzyme in human glucose metabolic pathway, we established a high throughput screening model for human pancreatic alpha-amylase inhibitors. METHODS: Pichia pastoris expression system was used to clone and express the human pancreatic alpha-amylase; we established the alpha-amylase inhibitor screening model using the catalytic properties of enzyme; this model was applied in screening of actinomycete' metabolites; the taxonomic status of positive strains were analyzed by constructing 16S rRNA phylogenetic tree. RESULTS: We cloned and expressed the intact gene of human pancreatic alpha-amylase successfully; the high-throughput screening model of alpha-amylase inhibitors was established; nearly 2000 actinomycete' metabolites were screened, 14 alpha-amylase inhibitor producing strains were obtained finally, and showed taxonomically rich diversity. CONCLUSION: The alpha-amylase inhibitor high-throughput screening model had high practical value for developing new hypoglycemic drugs.
Assuntos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Inibidores Enzimáticos / Alfa-Amilases Pancreáticas / Ensaios de Triagem em Larga Escala Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: Zh Revista: Wei Sheng Wu Xue Bao Ano de publicação: 2011 Tipo de documento: Article País de afiliação: China
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Inibidores Enzimáticos / Alfa-Amilases Pancreáticas / Ensaios de Triagem em Larga Escala Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: Zh Revista: Wei Sheng Wu Xue Bao Ano de publicação: 2011 Tipo de documento: Article País de afiliação: China