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Discrepancies in the P-glycoprotein-mediated transport of (18)F-MPPF: a pharmacokinetic study in mice and non-human primates.
Tournier, Nicolas; Cisternino, Salvatore; Peyronneau, Marie-Anne; Goutal, Sébastien; Dolle, Frédéric; Scherrmann, Jean-Michel; Bottlaender, Michel; Saba, Wadad; Valette, Héric.
Afiliação
  • Tournier N; CEA, I2BM, Service Hospitalier Frédéric Joliot, Orsay 91401, France. nicolas.tournier@cea.fr
Pharm Res ; 29(9): 2468-76, 2012 Sep.
Article em En | MEDLINE | ID: mdl-22644589
PURPOSE: Several in vivo studies have found that the 5-HT(1A) PET radioligand (18)F-MPPF is a substrate of rodent P-glycoprotein (P-gp). However, in vitro assays suggest that MPPF is not a substrate of human P-gp. We have now tested the influence of inhibiting P-gp on the brain kinetics of (18)F-MPPF in mice and non-human primates. METHODS: We measured the peripheral kinetics (arterial input function, metabolism, free fraction in plasma (f(P))) during (18)F-MPPF brain PET scanning in baboons with or without cyclosporine A (CsA) infusion. We measured (3)H-MPPF transport at the mouse BBB using in situ brain perfusion in P-gp/Bcrp deficient mice and after inhibiting P-gp with PSC833. RESULTS: There was an unexpected 1.9-fold increase in brain area under the curve in CsA-treated baboons (n = 4), with no change in radiometabolite-corrected arterial input. However, total volume of distribution corrected for f(P) (V(T)/f(P)) remained unchanged. In situ brain perfusion showed that P-gp restricted the permeability of the mouse BBB to (3)H-MPPF while Bcrp did not. CONCLUSION: These and previous in vitro results suggest that P-gp may not influence the permeability of human BBB to (18)F-MPPF. However, CsA treatment increased (18)F-MPPF free fraction, which is responsible for a misleading, P-gp unrelated enhanced brain uptake.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Radioisótopos de Flúor / Subfamília B de Transportador de Cassetes de Ligação de ATP Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Pharm Res Ano de publicação: 2012 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Radioisótopos de Flúor / Subfamília B de Transportador de Cassetes de Ligação de ATP Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Pharm Res Ano de publicação: 2012 Tipo de documento: Article País de afiliação: França