Lectin-binding sites in the anterior segment of the human eye.
Graefes Arch Clin Exp Ophthalmol
; 228(6): 528-32, 1990.
Article
em En
| MEDLINE
| ID: mdl-2265767
ABSTRACT
The anterior segment of the human eye was screened for differences in the lectin-binding patterns of Con A, PNA, GS-I, WGA, SBA, DBA, and UEA-I to enable cell typing for cell-culture purposes. An immunohistochemical technique combining an indirect antibody-lectin method with the avidin-biotin system was used. Con A and WGA were bound by all cells except the conjunctival goblet cells. UEA-I was exclusively bound by both vascular endothelial cells and some corneal and conjunctival epithelial cells. The binding of GS-I, PNA, SBA, and DBA showed an uneven pattern and differed among the cases investigated. The reasons for these differences are not clear. Our results indicate that the usefulness of lectins for cell-typing purposes is restricted and must be determined for every case.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lectinas
/
Segmento Anterior do Olho
Limite:
Aged
/
Aged80
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Graefes Arch Clin Exp Ophthalmol
Ano de publicação:
1990
Tipo de documento:
Article