The BEAF insulator regulates genes involved in cell polarity and neoplastic growth.
Dev Biol
; 369(1): 124-32, 2012 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-22743648
ABSTRACT
Boundary Element Associated Factor-32 (BEAF-32) is an insulator protein predominantly found near gene promoters and thought to play a role in gene expression. We find that mutations in BEAF-32 are lethal, show loss of epithelial morphology in imaginal discs and cause neoplastic growth defects. To investigate the molecular mechanisms underlying this phenotype, we carried out a genome-wide analysis of BEAF-32 localization in wing imaginal disc cells. Mutation of BEAF-32 results in miss-regulation of 3850 genes by at least 1.5-fold, 794 of which are bound by this protein in wing imaginal cells. Up-regulated genes encode proteins involved in cell polarity, cell proliferation and cell differentiation. Among the down-regulated genes are those encoding components of the wingless pathway, which is required for cell differentiation. Miss-regulation of these genes explains the unregulated cell growth and neoplastic phenotypes observed in imaginal tissues of BEAF-32 mutants.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação Neoplásica da Expressão Gênica
/
Polaridade Celular
/
Genes de Insetos
/
Proteínas de Drosophila
/
Elementos Isolantes
/
Proteínas de Ligação a DNA
/
Drosophila melanogaster
/
Proteínas do Olho
/
Neoplasias
Limite:
Animals
Idioma:
En
Revista:
Dev Biol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Estados Unidos