[Adenovirus mediated N19RhoA gene transfer attenuates neointimal formation in rats after carotid artery balloon injury].
Zhonghua Xin Xue Guan Bing Za Zhi
; 40(7): 601-6, 2012 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-22943691
ABSTRACT
OBJECTIVE:
To investigate the role of small G-protein RhoA in neointimal formation following rat carotid artery balloon injury and related mechanisms.METHODS:
Male 3-4-month-old Sprague-Dawley rats were used in the present study (10 rats per group). Group A control; Group B carotid artery balloon injury; Group C injury + Ad-CMV-eGFP + Pluronic F-127; Group D injury + Ad-CMV-N19RhoA-eGFP + Pluronic F-127; Group E non injury + Ad-CMV-eGFP + Pluronic F-127. Perivascular gene transfer of an adenovirus co-expressing N19RhoA was performed to rat carotid artery following balloon injury and the effect on neointimal formation and the expressions of PCNA and α-SM-actin examined. Rats were killed after 14 days.RESULTS:
The protein expression of RhoA in group B was significantly higher than in group A (P = 0.001), and the positive cells rate of PCNA and α-SM-actin which were assessed by immunohistochemistry in group C (45.2% and 75.6%) was significantly higher than in group D (28.4% and 51.9%, all P < 0.01). The area of neointima was significantly smaller [(0.14 ± 0.08) mm(2) vs. (0.23 ± 0.10) mm(2), P < 0.01], the luminal area was significantly larger [(0.47 ± 0.11) mm(2) vs. (0.31 ± 0.06) mm(2), P < 0.01] in group D than in group C.CONCLUSION:
Gene transfer of N19RhoA attenuates neointimal formation after balloon injury in rat carotid arteries possibly related to the modulating capacities of small G-protein RhoA on the proliferation, phenotypic differentiation and migration of vascular adventitial fibroblasts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lesões das Artérias Carótidas
/
Proteína rhoA de Ligação ao GTP
/
Neointima
Limite:
Animals
Idioma:
Zh
Revista:
Zhonghua Xin Xue Guan Bing Za Zhi
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
China