Complementary function of mitogen-activated protein kinase Hog1 from Trichosporonoides megachiliensis in Saccharomyces cerevisiae under hyper-osmotic stress.
J Biosci Bioeng
; 115(2): 127-32, 2013 Feb.
Article
em En
| MEDLINE
| ID: mdl-23063696
ABSTRACT
A (TmHog1) gene encoding a mitogen-activated protein kinase (MAPK) homologous to Saccharomyces cerevisiae Hog1 (ScHog1) involved in hyper-osmotic stress signaling was isolated from Trichosporonoides megachiliensis SN-124A, an erythritol-producing yeast. Although TmHog1, like other Hog1 homologs, encoded a kinase catalytic domain containing TGY motif, it was 50-60 amino acid residues shorter than the ScHog1. A TmHog1 transgene rescued the osmotic sensitivity and glycerol production defect of S. cerevisiae hog1Δ, a highly osmo-sensitive strain that does not produce glycerol, a compatible solute, during osmotic stress. Functional analyses of chimeric Hog1 proteins constructed from ScHog1 and TmHog1 sequences indicated that the C-terminal region of TmHog1 is more effective for glycerol biosynthesis than ScHog1 under osmotic stress.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ascomicetos
/
Saccharomyces cerevisiae
/
Proteínas Quinases Ativadas por Mitógeno
Idioma:
En
Revista:
J Biosci Bioeng
Assunto da revista:
ENGENHARIA BIOMEDICA
/
MICROBIOLOGIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Japão