Humans with chronic granulomatous disease maintain humoral immunologic memory despite low frequencies of circulating memory B cells.
Blood
; 120(24): 4850-8, 2012 Dec 06.
Article
em En
| MEDLINE
| ID: mdl-23074274
ABSTRACT
CD27(+) memory B cells are reduced in the blood of patients with chronic granulomatous disease (CGD) for reasons and consequences that remain unclear. Here we confirm not only decreased CD27(+) but also IgG(+) B cells in the blood of CGD patients compared with healthy donors (HDs). However, among IgG(+) B cells, the ratio of CD27(-) to CD27(+) was significantly higher in CGD patients compared with HDs. Similar to conventional memory B cells, CD27(-)IgG(+) B cells of CGD patients expressed activation markers and had undergone somatic hypermutation, albeit at levels lower than their CD27(+) counterparts. Functional analyses revealed slight reductions in frequencies of total IgG but not influenza-specific memory B-cell responses, as measured by Elispot in CGD patients compared with HDs. Serum IgG levels and influenza-specific antibodies were also normal in these CGD patients. Finally, we provide evidence that influenza-specific memory B cells can be present within the CD27(-)IgG(+) B-cell compartment. Together, these findings show that, despite reduced circulating CD27(+) memory B cells, CGD patients maintain an intact humoral immunologic memory, with potential contribution from CD27(-) B cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina G
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Linfócitos B
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Membro 7 da Superfamília de Receptores de Fatores de Necrose Tumoral
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Doença Granulomatosa Crônica
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Memória Imunológica
Limite:
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Blood
Ano de publicação:
2012
Tipo de documento:
Article