Phenolic profiling of Portuguese propolis by LC-MS spectrometry: uncommon propolis rich in flavonoid glycosides.
Phytochem Anal
; 24(4): 309-18, 2013.
Article
em En
| MEDLINE
| ID: mdl-23172843
ABSTRACT
INTRODUCTION:
Propolis is a chemically complex resinous substance collected by honeybees (Apis mellifera) from tree buds, comprising plant exudates, secreted substances from bee metabolism, pollen and waxes. Its chemical composition depends strongly on the plant sources available around the beehive, which have a direct impact in the quality and bioactivity of the propolis. Being as Portugal is a country of botanical diversity, the phenolic characterisation of propolis from the different regions is a priority.OBJECTIVE:
Extensive characterisation of the phenolic composition of Portuguese propolis from different continental regions and islands.METHOD:
Forty propolis ethanolic extracts were analysed extensively by liquid chromatography with diode-array detection coupled to electrospray ionisation tandem mass spectrometry (LC-DAD-ESI-MS(n) ).RESULTS:
Seventy-six polyphenols were detected in the samples and two groups of propolis were established the common temperate propolis, which contained the typical poplar phenolic compounds such as flavonoids and their methylated/esterified forms, phenylpropanoid acids and their esters, and an uncommon propolis type with an unusual composition in quercetin and kaempferol glycosides - some of them never described in propolis.CONCLUSION:
The method allowed the establishment of the phenolic profile of Portuguese propolis from different geographical locations, and the possibility to use some phenolic compounds, such as kaempferol-dimethylether, as geographical markers. Data suggest that other botanical species in addition to poplar trees can be important sources of resins for Portuguese propolis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Própole
/
Flavonoides
/
Cromatografia Líquida
/
Espectrometria de Massas por Ionização por Electrospray
País/Região como assunto:
Europa
Idioma:
En
Revista:
Phytochem Anal
Assunto da revista:
BOTANICA
/
QUIMICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Portugal