Paralog chromatography.
Biotechniques
; 8(2): 204-9, 1990 Feb.
Article
em En
| MEDLINE
| ID: mdl-2317377
ABSTRACT
As a mixed mode ligand, a small peptide can mimic an antibody's paratope (antigen recognition site). This report describes the construction of a representative set of paratope analogs, or "paralogs," which can be conjugated to a chromatographic sorbent to combine desirable characteristics of traditional high-performance liquid chromatography columns with the specificity of a moderate affinity antibody. The broad utility of this novel set of protein separatory reagents is illustrated on the complex mixture of proteins in a yeast lysate.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Fúngicas
/
Cromatografia
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
1990
Tipo de documento:
Article