Immunoaffinity studies on cationic peanut peroxidase fraction.
J Plant Physiol
; 116(4): 365-73, 1984.
Article
em En
| MEDLINE
| ID: mdl-23195306
ABSTRACT
Cationic peroxidase immobilized on sepharose has been used to separate specific antibodies from the antiperoxidase whole rabbit serum immunoglobulins (IgG) raised against it. The IgGs so separated completely pellet the peroxidase activity of the cationic peroxidase at a ratio of 6 1 (w/w). This shows the specificity of purified IgGs which is also shown by the single reaction arc obtained by immunoelectrophoresis of the isolated IgGs followed by challenging with crude peroxidase. These specific IgGs when linked to cyanogen bromide activated sepharose have been used to purify the cationic peroxidase from the spent medium proteins and from the cell extract of the peanut cells grown in suspension culture.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Plant Physiol
Assunto da revista:
BOTANICA
Ano de publicação:
1984
Tipo de documento:
Article